Soy beans

In the USDA Federal Grain Inspection Service's laboratory proficiency test "Biotechnology Proficiency Program April 2021", 125 laboratories worldwide participated to demonstrate their proficiency in identifying and quantifying GMO plants.

 

Our two laboratories AGROLAB LUFA in Kiel (Germany) and AGROLAB Italia in Vincenza (Italy) successfully participated in this global laboratory proficiency test. Different GMO maize and GMO soybean events were to be correctly identified and quantified in one sample per crop type. Of the 125 participants, 113 submitted their results on time.

The maize sample contained different combinations and concentrations of the following GMO maize events: T25, MON810, GA21, NK603, TC1507, MON863, DAS-59122-7, MIR604, Event 3272, MON 88017, MON 89034 and MIR 162.

The soy sample contained different combinations and concentrations of the following GMO soy events: Roundup Ready™ soy (GTS 40-3-2), Roundup Ready 2 Yield™ soy (MON89788), LibertyLink™ (A2704-12) soy and Plenish™ soy (DP-305423).

In a GMO screening, the presence of certain DNA sequences (regulatory sequence) that are very frequently used in different GMOs was to be tested.

In a second step, the GMO plants mentioned above were specifically tested for and their content quantitatively determined. Since there is a declaration threshold of 0.9 % GMO plant content in Europe, an exact quantification is necessary. Using real-time PCR, both laboratories achieved outstandingly correct values for all parameters, both qualitatively and quantitatively.

 

The following summarises the results for the maize tests (Table 1) for the soybean detections (Table 2) of the participants who used DNA-based methods:

 

Table 1
CORN

Results (total)

Thereof correct results

AGROLAB LUFA

AGROLAB Italia

35S

94

98,9%

Correct

Correct

NOS

91

96,7%

Correct

Correct

FMV

67

88,1%

Correct

Correct

T25

55

87,3%

Correct
(qual.)

Correct
(qual.)

MON 810

60

86,7%

Correct
(qual.)

Correct
(qual.)

GA 21

37

89,2%

Correct
(quant.)

Correct
(quant.)

NK 603

58

98,3%

Correct
(qual.)

Correct
(qual.)

Herculex (TC1507)

33

97,0%

Correct
(quant.)

Correct
(quant.)

MON 863

33

97,0%

not tested

Correct
(quant.)

Herculex RW (59122)

30

96,7%

Correct
(quant.)

Correct
(quant.)

MIR 604

56

96,4%

Correct
(qual.)

Correct
(qual.)

Event 3272

28

96,4%

not tested

not tested

MON 88017

34

91,2%

Correct
(quant.)

Correct
(quant.)

MON 89034

35

85,7%

Correct
(quant.)

Correct
(quant.)

MIR 162

35

97,1%

Correct
(quant.)

Correct
(quant.)

 

Table 2
SOYBEANS

Results (total)

Correct (total) %

AGROLAB LUFA

AGROLAB Italia

35S

73

98,6%

Correct

Correct

NOS

71

97,2%

Correct

Correct

FMV

60

96,7%

Correct

Correct

Roundup Ready®

28

92,9%

Correct
(quant.)

Correct
(quant.)

A2704-12 (Liberty Link®)

34

97,1%

Correct
(quant.)

Correct
(quant.)

Roundup Ready® II

31

100%

Correct
(quant.)

Correct
(quant.)

DP305423

55

98,2%

Correct
(qual.)

Correct
(qual.)

 

YOUR PLUS: In recent years, AGROLAB has continuously expanded its portfolio of qualitative and quantitative detections for genetic modifications and offers accredited GMO testing in plant raw materials, feed and food. Ask for an offer tailored to your needs.

 

Links: 

  1. product information GMO analysis
  2. GMO legal requirements


 
  

Authors: Dr. Frank Mörsberger, Anja Palisch

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